|
KMID : 0613820080180060839
|
|
Journal of Life Science
2008 Volume.18 No. 6 p.839 ~ p.844
|
|
|
Optimal Culture Conditions on the Keratinase Production by Bacillus sp. SH-517
|
|
Bang Byung-Ho
Rhee Moon-Soo
Lim Ki-Hwan
Yi Dong-Heui
|
|
|
Abstract
|
|
|
|
A strain SH-517 which produce extracellular keratinase, was isolated from the soil of a poultry waste and a poultry factory. An isolate SH-517 was identified as Bacillus sp. based on its morphological and biochemical characteristics. The optimal culture conditions for the production of keratinase by Bacillus sp. SH-517 were investigated. The optimal medium composition for keratinase production was determined to be 2.0% chicken feather as carbon source, 0.5% beef extract as organic nitrogen source, 0.5% KNO₃ as inorganic nitrogen source and 0.06% KCl, 0.05% NaCl, 0.04% KH₂PO₄, 0.03% K₂HPO₄ as mineral source and 0.01% yeast extract as growth factor. The optimal temperature and pH of medium were shown 40℃ and 8.5 with shaking culture (180 rpm/min), respectively. The maximum keratinase production reached maximum of 125 units/ml/min after 42 hr of cultivation under the optimal culturing conditions.
|
|
|
KEYWORD
|
|
|
Keratinase, chickenfeather, production, Bacillussp.SH-517
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|